ꯐꯨꯠ ꯑꯃꯁꯨꯡ ꯃꯥꯎꯊ ꯗꯤꯖꯤꯖ ꯚꯥꯏꯔꯁ-o ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯑꯦꯂꯤꯁꯥ ꯀꯤꯠ

ꯁ꯭ꯄꯦꯁꯤꯐꯤꯀꯦꯁꯟ ꯇꯧꯕꯥ

fmdv-o ꯑꯦ.ꯕꯤ.

ꯁꯤꯖꯤꯟꯅꯅꯕꯥ ꯄꯥꯟꯗꯝ ꯊꯝꯂꯤꯕꯥ .: ꯕꯣꯋꯤꯟ ꯐꯨꯠ ꯑꯃꯁꯨꯡ ꯃꯥꯎꯊ ꯗꯤꯖꯤꯖ ꯚꯥꯏꯔꯁ-o .

ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯑꯦꯂꯤꯁꯥ ꯗꯤꯇꯦꯛꯁꯟ ꯀꯤꯠ ꯑꯁꯤ ꯏꯟ ꯚꯤꯠꯔꯣꯗꯥ ꯕꯣꯋꯤꯅꯒꯤ ꯁꯦꯔꯥꯃꯗꯥ ꯈꯣꯡ ꯑꯃꯁꯨꯡ ꯃꯎꯊ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯂꯦꯄꯊꯣꯀꯄꯅꯤ꯫ ꯇꯦꯁ꯭ꯠ ꯑꯁꯤ ꯐꯥꯔꯃꯁꯤꯡꯒꯤ ꯈꯣꯡ ꯑꯃꯁꯨꯡ ꯃꯥꯎꯊ ꯗꯤꯖꯤꯖ ꯚꯥꯏꯔꯁ ꯃꯈꯜ o ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯏꯝꯝꯌꯨꯅꯤꯇꯤ ꯁ꯭ꯇꯦꯇꯁ ꯑꯁꯤ ꯌꯦꯡꯁꯤꯟꯅꯕꯥ ꯁꯤꯖꯤꯟꯅꯩ꯫

ꯁ꯭ꯄꯦꯁꯤꯃꯦꯟ ꯴.: ꯁꯦꯔꯥꯝ .

ꯃꯊꯧ ꯇꯥꯕꯥ ꯑꯗꯨꯕꯨ ꯄꯤꯗꯕꯥ ꯃꯆꯥꯀꯁꯤꯡ:

ꯁꯨꯡꯗꯣꯛꯂꯕꯥ ꯏꯁꯤꯡ

ꯄꯥꯏꯄꯦꯇꯔꯅꯥ ꯌꯨ.ꯑꯦꯁ.

꯹꯶-ꯋꯦꯜ ꯃꯥꯏꯛꯔꯣꯞꯂꯦꯠ ꯔꯤꯗꯔ (꯴꯵꯰ꯑꯦꯟ.ꯑꯦꯝ.)

ꯇꯥꯏꯃꯔ ꯴.

ꯁꯦꯟꯠꯔꯦꯐ꯭ꯌꯨꯖ (꯴꯰꯰꯰ꯑꯥꯔ.ꯄꯤ.ꯑꯦꯝ.) ꯑꯣꯏꯕꯥ ꯌꯥꯏ꯫

ꯋꯥꯁꯤꯡ ꯃꯦꯁꯤꯟ

ꯑꯣꯚꯦꯟ (꯳꯷ ꯗꯤꯒ꯭ꯔꯤ ) ꯑꯃꯁꯨꯡ ꯑꯦꯟ.

ꯇꯦꯁ꯭ꯇꯀꯤ ꯊꯧꯑꯣꯡꯁꯤꯡ & ꯏꯟꯇꯔꯞꯔꯤꯇꯦꯁꯟ .

ꯈꯣꯡꯊꯥꯡ ꯱ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯒꯤ ꯊꯧꯑꯣꯡ

꯱.꯱ ꯋꯥꯁ ꯁꯣꯜꯌꯨꯁꯟ ꯁꯦꯝ ꯁꯥꯕꯥ .

Wꯑꯣꯔꯀꯤꯡ ꯀꯟꯁꯦꯟꯠꯔꯦꯁꯟ . wꯑꯦꯁꯤꯡ ꯂꯤꯛꯌꯨꯏꯗ: ꯱.ꯁꯤꯖꯤꯟꯅꯗ꯭ꯔꯤꯉꯩ ꯃꯃꯥꯡꯗꯥ, ꯋꯥꯁ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯗꯨ ꯔꯨꯝ ꯇꯦꯝꯄꯦꯔꯦꯆꯔ (20-25 ꯗꯤꯒ꯭ꯔꯤ )ꯗꯥ ꯑꯃꯨꯛ ꯍꯟꯅꯥ ꯊꯃꯒꯗꯕꯅꯤ ꯑꯃꯁꯨꯡ ꯃꯗꯨ ꯌꯨꯅꯤꯐꯣꯔꯝ ꯑꯣꯏꯍꯟꯅꯕꯥ ꯆꯥꯡ ꯃꯥꯟꯅꯅꯥ ꯁꯦꯃꯖꯤꯅꯒꯗꯕꯅꯤ꯫ ꯃꯗꯨꯒꯤ ꯃꯇꯨꯡꯗꯥ ꯀꯟꯁꯦꯟꯠꯔꯦꯇꯦꯗ ꯋꯥꯁ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯗꯨ ꯁꯨꯡꯗꯣꯛꯂꯕꯥ ꯏꯁꯤꯡꯅꯥ 20 ꯔꯛ ꯊꯀꯎ (ꯈꯨꯗꯝ ꯑꯣꯏꯅꯥ, 5ml ꯀꯟꯁꯦꯟꯠꯔꯦꯇꯦꯗ ꯋꯥꯁꯤꯡ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯗꯨ 95ml ꯁꯨꯡꯗꯣꯛꯂꯕꯥ ꯏꯁꯤꯡꯗꯥ ꯍꯥꯄꯆꯤꯜꯂꯤ), ꯃꯗꯨ ꯌꯥꯝꯅꯥ ꯊꯨꯅꯥ ꯌꯥꯟꯁꯤꯟꯅꯧ, ꯑꯃꯁꯨꯡ ꯂꯨꯄꯊꯣꯛꯂꯕꯥ ꯋꯥꯁꯛ ꯑꯗꯨ ꯅꯨꯃꯤꯠ 7ꯅꯤꯒꯤ ꯑꯣꯏꯅꯥ ꯗꯤꯒ꯭ꯔꯤ 2-8ꯗꯥ ꯊꯝꯕꯥ ꯌꯥꯏ꯫

꯱.꯲ ꯁꯦꯝꯄꯜ ꯁꯦꯝ ꯁꯥꯕꯥ .

2 ꯃꯄꯨꯡ ꯑꯣꯏꯕꯥ ꯁꯥꯒꯤ ꯏ ꯂꯧꯈꯤ, ꯃꯤꯅꯠ 10ꯒꯤ ꯑꯣꯏꯅꯥ 4000rpmꯗꯥ ꯁꯦꯟꯠꯔꯦꯐ꯭ꯌꯨꯖ ꯇꯧꯈꯤ, ꯑꯃꯁꯨꯡ ꯁꯨꯄꯔꯅꯦꯇꯦꯟꯇ ꯑꯗꯨ ꯈꯣꯃꯖꯤꯅꯈꯤ꯫ ꯁꯦꯔꯥꯝ ꯑꯁꯤ ꯃꯍꯧꯁꯥꯅꯥ ꯍꯦꯃꯣꯂꯥꯏꯁꯤꯁ ꯌꯥꯎꯗꯅꯥ ꯀꯣꯑꯦꯒꯨꯂꯦꯠ ꯇꯧꯕꯥ ꯌꯥꯏ꯫

2 ꯁꯦꯔꯥꯝ ꯁꯦꯝꯄꯜ 1:25 (5UL ꯁꯦꯔꯥꯝ ꯁꯦꯝꯄꯜ ꯑꯗꯨ 120UL ꯁꯦꯝꯄꯜ ꯗꯤꯜꯌꯨꯑꯦꯟꯇ ꯑꯗꯨꯗꯥ ꯍꯥꯄꯆꯤꯜꯂꯨ) ꯑꯃꯁꯨꯡ ꯃꯄꯨꯡꯐꯥꯅꯥ ꯌꯥꯟꯁꯤꯟꯅꯧ꯫

ꯅꯣꯠ: ꯅꯦꯒꯦꯇꯤꯚ ꯑꯃꯁꯨꯡ ꯄꯣꯖꯤꯇꯤꯚ ꯀꯟꯠꯔꯣꯂꯁꯤꯡꯗꯁꯨ ꯗꯤꯜꯌꯨꯠ ꯃꯊꯧ ꯇꯥꯏ꯫

꯱.꯳ ꯑꯦꯅꯖꯥꯏꯝ ꯀꯟꯖꯨꯒꯦꯠ ꯁꯦꯝ ꯁꯥꯕꯥ .

ꯑꯦꯅꯖꯥꯏꯝ ꯀꯟꯖꯨꯒꯦꯠ 1:90 (ꯑꯦꯅꯖꯥꯏꯝ ꯀꯟꯖꯨꯒꯦꯠ(X100) ꯑꯗꯨ 445UL ꯁꯦꯝꯄꯜ ꯗꯤꯜꯌꯨꯑꯦꯟꯇ ꯐꯥꯎꯕꯥ ꯌꯥꯎꯍꯜꯂꯨ ꯑꯃꯁꯨꯡ ꯃꯄꯨꯡꯐꯥꯅꯥ ꯌꯥꯟꯁꯤꯟꯅꯧ꯫

꯱.꯴ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯒꯤ ꯊꯧꯑꯣꯡ

4 ꯑꯦꯅꯖꯥꯏꯝ ꯂꯦꯕꯦꯜ ꯃꯥꯏꯛꯔꯣꯞꯂꯦꯠ (ꯁꯦꯝꯄꯜ ꯃꯁꯤꯡꯒꯤ ꯃꯇꯨꯡ ꯏꯟꯅꯥ, ꯗꯤꯁꯑꯦꯕꯜ ꯇꯧꯕꯥ ꯌꯥꯏ ꯑꯃꯁꯨꯡ ꯀꯌꯥꯃꯔꯨꯝ ꯁꯤꯖꯤꯟꯅꯕꯥ ꯌꯥꯏ), ꯑꯦꯅꯖꯥꯏꯝ ꯂꯦꯕꯦꯜ ꯃꯥꯏꯛꯔꯣꯞꯂꯦꯠꯇꯥ ꯊꯥꯎ ꯊꯣꯀꯄꯥ ꯁꯦꯔꯥꯝ 100ul ꯍꯥꯄꯆꯤꯟꯗꯨꯅꯥ, ꯅꯦꯒꯦꯇꯤꯚ ꯑꯃꯁꯨꯡ ꯄꯣꯖꯤꯇꯤꯚ ꯁꯦꯝꯄꯂꯁꯤꯡꯒꯤꯗꯃꯛ ꯋꯦꯜ ꯑꯅꯤ ꯑꯃꯃꯝ ꯊꯝꯃꯨ, ꯑꯃꯁꯨꯡ ꯅꯦꯒꯦꯇꯤꯚ ꯑꯃꯁꯨꯡ ꯄꯣꯖꯤꯇꯤꯚ ꯑꯣꯏꯕꯥ ꯀꯟꯠꯔꯣꯂꯁꯤꯡꯒꯤꯗꯃꯛꯇꯥ ꯃꯊꯪꯁꯤꯠꯅꯥ 100UL ꯑꯃꯥ ꯔꯤꯑꯦꯛꯁꯟ ꯋꯦꯂꯗꯥ ꯍꯥꯄꯆꯤꯜꯂꯨ꯫ ꯁꯦꯝꯄꯜ ꯑꯗꯨ ꯇꯞꯅꯥ ꯇꯞꯅꯥ ꯌꯥꯟꯁꯤꯟꯅꯗꯨꯅꯥ, ꯄ꯭ꯂꯦꯠ ꯑꯗꯨ ꯀꯨꯄꯁꯤꯟꯅꯕꯥ ꯁꯤꯂꯤꯡ ꯄ꯭ꯂꯦꯠ ꯐꯤꯜꯃ ꯑꯗꯨ ꯁꯤꯖꯤꯟꯅꯗꯨꯅꯥ, ꯑꯃꯁꯨꯡ ꯃꯤꯅꯠ 30ꯒꯤ ꯑꯣꯏꯅꯥ ꯗꯤꯒ꯭ꯔꯤ 37ꯗꯥ ꯏꯅꯀꯨꯕꯦꯁꯟ ꯇꯧ꯫

2 ꯁꯤꯂꯤꯡ ꯄ꯭ꯂꯦꯠ ꯐꯤꯜꯃ ꯑꯗꯨ ꯍꯥꯡꯗꯣꯀꯎ, ꯄ꯭ꯂꯦꯠ ꯑꯗꯨꯗꯥ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯗꯨ ꯀꯨꯄꯁꯤꯜꯂꯨ, ꯋꯦꯜ ꯈꯨꯗꯤꯡꯃꯛꯇꯥ ꯋꯥꯔꯀꯤꯡ ꯀꯟꯁꯦꯟꯠꯔꯦꯁꯟ ꯋꯥꯁꯤꯡ ꯂꯤꯛꯌꯨꯏꯗ 300μl ꯍꯥꯄꯆꯤꯜꯂꯨ, ꯑꯃꯁꯨꯡ ꯃꯗꯨꯒꯤ ꯃꯇꯨꯡꯗꯥ ꯂꯤꯛꯌꯨꯏꯗ ꯑꯗꯨ ꯀꯨꯌꯣꯝ ꯑꯗꯨꯗꯥ ꯊꯥꯗꯣꯀꯎ꯫ ꯃꯊꯛꯀꯤ ꯁ꯭ꯇꯦꯄꯁꯤꯡ ꯑꯗꯨ 5 ꯁꯨꯕꯥ ꯑꯣꯏꯅꯥ ꯍꯟꯖꯤꯟ ꯍꯟꯖꯤꯟ ꯇꯧ, ꯑꯃꯁꯨꯡ ꯑꯔꯣꯏꯕꯗꯥ ꯑꯁꯦꯡꯕꯥ ꯑꯦꯕꯁꯣꯔꯕꯦꯟꯇ ꯄꯦꯄꯔ ꯑꯃꯗꯥ ꯂꯦꯡꯗꯅꯥ ꯂꯩꯌꯨ꯫

3 ꯋꯦꯜ ꯈꯨꯗꯤꯡꯃꯛꯇꯥ ꯑꯦꯅꯖꯥꯏꯝ ꯀꯟꯖꯨꯒꯦꯠ 100μl ꯌꯥꯎꯍꯜꯂꯨ, ꯄ꯭ꯂꯦꯠ ꯑꯗꯨ ꯀꯨꯄꯁꯤꯜꯂꯨ ꯑꯃꯁꯨꯡ ꯃꯤꯅꯠ 45ꯒꯤ ꯑꯣꯏꯅꯥ ꯗꯤꯒ꯭ꯔꯤ 37ꯗꯥ ꯏꯅꯛꯌꯨꯕꯦꯠ ꯇꯧ꯫

1 ꯁꯤꯂꯤꯡ ꯄ꯭ꯂꯦꯠ ꯐꯤꯜꯃ ꯑꯗꯨ ꯍꯥꯡꯗꯣꯀꯎ, ꯄ꯭ꯂꯦꯠ ꯑꯗꯨꯗꯥ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯗꯨ ꯀꯨꯄꯁꯤꯜꯂꯨ, ꯋꯦꯜ ꯈꯨꯗꯤꯡꯃꯛꯇꯥ ꯋꯥꯔꯀꯤꯡ ꯀꯟꯁꯦꯟꯠꯔꯦꯁꯟ ꯋꯥꯁꯤꯡ ꯂꯤꯛꯌꯨꯏꯗ 300μl ꯍꯥꯄꯆꯤꯜꯂꯨ, ꯑꯃꯁꯨꯡ ꯃꯗꯨꯒꯤ ꯃꯇꯨꯡꯗꯥ ꯂꯤꯛꯌꯨꯏꯗ ꯑꯗꯨ ꯀꯨꯌꯣꯝ ꯑꯗꯨꯗꯥ ꯊꯥꯗꯣꯀꯎ꯫ ꯃꯊꯛꯀꯤ ꯁ꯭ꯇꯦꯄꯁꯤꯡ ꯑꯗꯨ 5 ꯁꯨꯕꯥ ꯑꯣꯏꯅꯥ ꯍꯟꯖꯤꯟ ꯍꯟꯖꯤꯟ ꯇꯧ, ꯑꯃꯁꯨꯡ ꯑꯔꯣꯏꯕꯗꯥ ꯑꯁꯦꯡꯕꯥ ꯑꯦꯕꯁꯣꯔꯕꯦꯟꯇ ꯄꯦꯄꯔ ꯑꯃꯗꯥ ꯂꯦꯡꯗꯅꯥ ꯂꯩꯌꯨ꯫

100μl ꯁꯕꯁ꯭ꯠꯔꯦꯠ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯃꯥ ꯌꯥꯟꯁꯤꯟꯅꯗꯨꯅꯥ, ꯄ꯭ꯂꯦꯠ ꯑꯗꯨ ꯀꯀꯊꯠꯂꯨ ꯑꯃꯁꯨꯡ ꯃꯩꯁꯥꯗꯒꯤ ꯂꯥꯞꯅꯥ ꯂꯩꯕꯥ ꯗꯤꯒ꯭ꯔꯤ 37ꯗꯥ ꯃꯤꯅꯠ 10 ꯊꯃꯒꯗꯕꯅꯤ꯫

2 50μl ꯁ꯭ꯇꯞ ꯁꯣꯜꯌꯨꯁꯟ ꯑꯃꯗꯥ ꯌꯥꯟꯁꯤꯟꯅꯧ, ꯑꯃꯁꯨꯡ ꯔꯤꯖꯜꯇ ꯑꯗꯨ ꯃꯤꯅꯠ 5ꯒꯤ ꯃꯅꯨꯡꯗꯥ ꯂꯦꯄꯀꯅꯨ꯫

ꯈꯣꯡꯊꯥꯡ ꯲ꯒꯤ ꯔꯤꯖꯜꯇ ꯏꯟꯇꯔꯞꯔꯤꯇꯦꯁꯟ .

ꯋꯦꯜ ꯈꯨꯗꯤꯡꯃꯛꯀꯤ ꯑꯣ.ꯗꯤ.꯴꯵꯰ ꯚꯦꯜꯌꯨ ꯑꯁꯤ ꯹꯶-ꯋꯦꯜ ꯃꯥꯏꯛꯔꯣꯞꯂꯦꯠ ꯔꯤꯗꯔꯗꯥ ꯄꯥꯈꯤ꯫

꯲.꯱ ꯚꯦꯂꯤꯗꯦꯁꯟ ꯇꯧꯕꯥ .

ꯅꯦꯒꯦꯇꯤꯕ ꯀꯟꯠꯔꯣꯂꯒꯤ ꯆꯥꯡꯆꯠꯇꯥ ꯑꯣꯏꯕꯥ ꯑꯣ.ꯗꯤ.꯴꯵꯰ꯑꯦꯟ.ꯑꯦꯝ.<0.15 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.

꯲.꯲ꯒꯤ ꯍꯤꯁꯥꯕ ꯇꯧꯕꯒꯤ ꯃꯑꯣꯡ .

ꯁꯦꯝꯄꯜ ꯈꯨꯗꯤꯡꯃꯛꯀꯤ ꯑꯣꯏꯅꯥ, ꯀꯣꯔꯤꯑꯦꯛꯇ ꯇꯧꯔꯕꯥ ꯁꯦꯝꯄꯜ ꯑꯃꯁꯨꯡ ꯀꯟꯠꯔꯣꯜ ꯚꯦꯜꯌꯨꯁꯤꯡ ꯁꯤꯖꯤꯟꯅꯗꯨꯅꯥ ꯃꯈꯥꯗꯥ ꯄꯤꯔꯤꯕꯥ S/P ꯑꯁꯤ ꯂꯦꯄꯀꯅꯨ:

꯲.꯳ ꯔꯤꯖꯜꯇ ꯏꯟꯇꯔꯞꯔꯤꯇꯦꯁꯟ ꯇꯧꯕꯥ

ꯑꯦꯁ/ꯄꯤ ꯇꯧꯕꯥ ꯃꯇꯃꯗꯥ .<0.15, it is judged as negative.

S/Pꯅꯥ 0.25ꯗꯒꯤ ꯍꯦꯟꯅꯥ ꯅꯠꯠꯔꯒꯥ ꯃꯥꯟꯅꯕꯥ ꯃꯇꯃꯗꯥ, ꯃꯁꯤ ꯄꯣꯖꯤꯇꯤꯚ ꯑꯣꯏꯅꯥ ꯂꯧꯏ꯫

ꯑꯦꯁ/ꯄꯤꯗꯒꯤ ꯍꯦꯟꯅꯥ ꯅꯦꯝꯕꯥ ꯅꯠꯠꯔꯒꯥ ꯃꯥꯟꯅꯕꯥ ꯃꯇꯃꯗꯥ 0.15 .<0.25, it is judged as suspicious, and suggest to repeat sampling and test one week later.

ꯍꯠ ꯇꯦꯒꯁ: ꯐꯨꯠ ꯑꯃꯁꯨꯡ ꯃꯥꯎꯊ ꯗꯤꯖꯤꯖ ꯚꯥꯏꯔꯁ-O ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯑꯦꯂꯤꯁꯥ ꯀꯤꯠ, ꯆꯥꯏꯅꯥ ꯐꯨꯠ ꯑꯃꯁꯨꯡ ꯃꯥꯎꯊ ꯗꯤꯖꯤꯖ ꯚꯥꯏꯔꯁ-o ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯑꯦꯂꯤꯁꯥ ꯀꯤꯠ ꯁꯞꯂꯥꯏꯌꯔꯁꯤꯡ, ꯃꯦꯟꯌꯨꯐꯦꯀꯆꯔꯔꯁꯤꯡ, ꯐꯦꯛꯇꯔꯤ