ꯕꯣꯋꯤꯟ ꯇ꯭ꯌꯨꯕꯔꯀꯨꯂꯣꯁꯤꯁ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯥ

ꯁ꯭ꯄꯦꯁꯤꯐꯤꯀꯦꯁꯟ ꯇꯧꯕꯥ

ꯕꯣꯋꯤꯟ ꯇ꯭ꯌꯨꯕꯔꯀꯨꯂꯣꯁꯤꯁ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯥ

ꯁꯤꯖꯤꯟꯅꯅꯕꯥ ꯄꯥꯟꯗꯝ ꯊꯝꯂꯤꯕꯥ .: ꯁꯟ-ꯏꯔꯣꯏꯒꯤ ꯁꯦꯔꯥꯝ, ꯄ꯭ꯂꯥꯖꯃꯥ ꯅꯠꯠꯔꯒꯥ ꯍꯣꯜ ꯕ꯭ꯂꯗꯇꯥ ꯕꯣꯋꯤꯟ ꯇ꯭ꯌꯨꯕꯔꯀꯨꯂꯣꯁꯤꯁ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ (BTB AB)ꯒꯤ ꯀ꯭ꯕꯥꯂꯤꯇꯦꯇꯤꯚ ꯗꯤꯇꯦꯛꯁꯅꯒꯤꯗꯃꯛꯇꯥ꯫ ꯃꯁꯤꯒꯤ ꯑꯦꯁꯦꯁ ꯑꯁꯤꯗꯥ ꯑꯦꯝ.ꯄꯤ.ꯕꯤ.꯷꯰ ꯑꯃꯁꯨꯡ ꯑꯦꯝ.ꯄꯤ.ꯕꯤ.꯸꯳ ꯆꯥꯏꯃꯦꯔꯥ ꯔꯤꯀꯣꯝꯕꯤꯅꯦꯟꯇ ꯁꯤꯖꯤꯟꯅꯈꯤ꯫

ꯄ꯭ꯔꯤꯟꯁꯤꯄꯂꯒꯤ ꯃꯇꯨꯡ ꯏꯟꯅꯥ .: ꯂꯦꯇꯔꯜ ꯐ꯭ꯂꯣ ꯏꯝꯝꯌꯨꯅꯣꯛꯔꯣꯃꯦꯇꯣꯒ꯭ꯔꯥꯐꯤꯛ ꯑꯦꯁꯦꯁ꯫

ꯁ꯭ꯄꯦꯁꯤꯃꯦꯟ ꯴.: ꯁꯦꯔꯥꯝ, ꯄ꯭ꯂꯥꯖꯃꯥ ꯅꯠꯔꯒꯥ ꯍꯣꯜ ꯕ꯭ꯂꯗ .

ꯄꯤꯔꯤꯕꯥ ꯃꯆꯥꯀꯁꯤꯡ ꯑꯁꯤ .:

꯱꯰ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯥ ꯄꯥꯎꯆꯦꯁꯤꯡ, ꯀꯥꯔꯗ ꯑꯃꯁꯨꯡ ꯗꯦꯁꯤꯀꯦꯟꯇꯁꯤꯡꯒꯥ ꯂꯣꯌꯅꯅꯥ

ꯀꯦꯄꯤꯂꯦꯔꯤ ꯗ꯭ꯔꯣꯄꯔ ꯱꯰ ꯌꯥꯑꯣꯔꯤ꯫

꯱ ꯕꯣꯇꯜ ꯑꯣꯐ ꯑꯦꯁꯦꯁ ꯕꯐꯔ

꯱ ꯄꯦꯀꯦꯖ ꯏꯅꯁꯥꯔꯠ ꯇꯧꯕꯥ ꯌꯥꯏ꯫

ꯑꯈꯟꯅꯕꯥ ꯐꯤꯆꯔꯁꯤꯡ:

꯱.ꯍꯥꯏ ꯁꯦꯟꯁꯤꯇꯤꯕꯤꯇꯤ ꯑꯃꯁꯨꯡ ꯑꯦꯛꯌꯨꯔꯦꯁꯤ꯫

2.ꯋꯥꯟ-ꯁ꯭ꯇꯦꯞ ꯇꯦꯁ꯭ꯇꯤꯡ ꯄ꯭ꯔꯣꯁꯤꯖꯔ

꯳.ꯑꯆꯨꯝꯕꯥ ꯐꯂꯁꯤꯡ .

ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯒꯤ ꯊꯧꯑꯣꯡ ꯑꯁꯤ .

-ꯁꯥꯒꯤ ꯑꯅꯧꯕꯥ ꯍꯣꯜ ꯕ꯭ꯂꯗ ꯈꯣꯃꯖꯤꯜꯂꯨ, ꯑꯃꯁꯨꯡ ꯁꯤꯖꯤꯟꯅꯅꯕꯥ ꯁꯦꯔꯥꯝ ꯅꯠꯠꯔꯒꯥ ꯄ꯭ꯂꯥꯖꯃꯥ ꯁ꯭ꯄꯦꯁꯤꯃꯦꯟ ꯑꯗꯨ ꯂꯧ꯫ ꯁ꯭ꯄꯦꯁꯤꯃꯦꯟ ꯑꯗꯨ ꯈꯣꯃꯖꯤꯜꯂꯕꯥ ꯃꯇꯨꯡꯗꯥ ꯇꯦꯁ꯭ꯇꯤꯡ ꯑꯗꯨ ꯈꯨꯗꯛꯇꯥ ꯁꯤꯖꯤꯟꯅꯧ꯫

-ꯐꯣꯏꯜ ꯄꯥꯎꯆꯦꯗꯒꯤ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯥ ꯗꯤꯚꯥꯏꯁ ꯑꯗꯨ ꯂꯧꯊꯣꯀꯎ ꯑꯃꯁꯨꯡ ꯃꯗꯨ ꯍꯣꯔꯥꯏꯖꯣꯟꯇꯦꯜ ꯑꯣꯏꯅꯥ ꯊꯝꯃꯨ꯫

-ꯀꯦꯄꯤꯂꯦꯔꯤ ꯗ꯭ꯔꯣꯄꯔ ꯑꯁꯤ ꯇꯦꯁ꯭ꯠ ꯇꯧꯕꯥ ꯗꯤꯚꯥꯏꯁꯀꯤ ꯁꯦꯝꯄꯜ ꯋꯦꯜ "s"ꯗꯥ ꯁꯦꯝ ꯁꯥꯕꯥ ꯁ꯭ꯄꯦꯁꯤꯃꯦꯟ 10μl ꯊꯝꯅꯕꯥ꯫ ꯃꯗꯨꯒꯤ ꯃꯇꯨꯡꯗꯥ ꯈꯨꯗꯛꯇꯥ ꯁꯦꯝꯄꯜ ꯑꯗꯨꯗꯥ ꯌꯥꯝꯅꯥ ꯊꯨꯅꯥ ꯌꯥꯟꯁꯤꯟꯅꯔꯕꯥ ꯑꯦꯁꯦ ꯕꯐꯔ ꯑꯗꯨꯒꯤ ꯗ꯭ꯔꯣꯞ 3 ꯊꯥꯎ꯫

-ꯔꯤꯖꯜ ꯑꯗꯨ ꯃꯤꯅꯤꯠ 5-10ꯗꯥ ꯏꯟꯇꯔꯞꯔꯤꯠ ꯇꯧ꯫ ꯃꯤꯅꯠ 15ꯒꯤ ꯃꯇꯨꯡꯗꯥ ꯔꯤꯖꯜꯇ ꯑꯗꯨ ꯏꯅꯚꯦꯂꯤꯗ ꯑꯣꯏꯅꯥ ꯂꯧꯏ꯫

ꯔꯤꯖꯜꯇꯁꯤꯡꯒꯤ ꯏꯟꯇꯔꯞꯔꯤꯇꯦꯁꯟ ꯇꯧꯕꯥ

ꯄꯣꯖꯤꯇꯤꯕ (+): "ꯁꯤ" ꯂꯥꯏꯟ ꯑꯃꯁꯨꯡ ꯖꯣꯟ "ꯇꯤ" ꯂꯥꯏꯟ ꯑꯅꯤꯃꯛ ꯂꯩꯕꯥ, ꯇꯤ ꯂꯥꯏꯟ ꯑꯁꯤ ꯃꯌꯦꯛ ꯁꯦꯡꯅꯥ ꯅꯠꯠꯔꯒꯥ ꯃꯌꯦꯛ ꯁꯦꯡꯗꯕꯥ ꯑꯣꯏꯔꯕꯁꯨ꯫

ꯅꯦꯒꯦꯇꯤꯕ ({0}}): ꯀ꯭ꯂꯤꯌꯥꯔ ꯁꯤ ꯂꯥꯏꯟ ꯈꯛꯇꯃꯛ ꯎꯕꯥ ꯐꯪꯒꯅꯤ꯫ ꯅꯣ ꯇꯤ ꯂꯥꯏꯟ꯫

ꯑꯁꯣꯌꯕꯥ: ꯁꯤ ꯖꯣꯅꯗꯥ ꯃꯆꯨꯒꯤ ꯂꯥꯏꯟ ꯑꯃꯠꯇꯥ ꯌꯥꯑꯣꯗꯦ꯫ ꯀꯔꯤꯒꯨꯝꯕꯥ ꯇꯤ ꯂꯥꯏꯟ ꯑꯃꯥ ꯂꯥꯀꯄꯥ ꯃꯇꯃꯗꯥ ꯀꯔꯤꯒꯨꯝꯕꯥ ꯑꯃꯠꯇꯥ ꯂꯩꯇꯦ꯫

ꯑꯣꯔꯗꯔꯒꯤ ꯃꯇꯥꯡꯗꯥ ꯏꯅꯐꯣꯔꯃꯦꯁꯟ .

ꯍꯠ ꯇꯦꯒꯁ: ꯕꯣꯋꯤꯟ ꯇ꯭ꯌꯨꯕꯔꯀꯨꯂꯣꯁꯤꯁ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯇꯦꯁ꯭ꯠ, ꯆꯥꯏꯅꯥ ꯕꯣꯋꯤꯟ ꯇ꯭ꯌꯨꯕꯔꯀꯨꯂꯣꯁꯤꯁ ꯑꯦꯟꯇꯤꯕꯣꯗꯤ ꯇꯦꯁ꯭ꯠ ꯁꯞꯂꯥꯏꯌꯔꯁꯤꯡ, ꯃꯦꯟꯌꯨꯐꯦꯀꯆꯔꯔꯁꯤꯡ, ꯐꯦꯛꯇꯔꯤ